MRL also helped in the mobile phase preparation during the HPLC study.
The present study has developed a much simpler HPLC method without incurring expensive chemicals and laborious mobile phase preparation.
All solvents used for mobile phase preparation were Optima LCMS grade and were manufactured by Fisher Scientific.
This method saves time in terms of mobile phase preparation and cuts cost by excluding additional purchase of expensive chemicals.
HPLC on the other hand involves costly chemicals and tedious mobile phase preparation although it can offer a much lower LOD and better consistency.
It was concluded that mobile phase preparation was found to be stable for about 7 days since aged mobile phase produced equivalent chromatography and results to that obtained with fresh mobile phase.
MRL performed the wet lab study of collecting the dilutions of the sample after stress conditions for the drug sample and helped in the mobile phase preparation during the HPLC study.
The method was validated as per ICH Q2 (R1); the proposed ion chromatography method is found to be simple, sensitive, accurate, precise, specific and green chemistry type of analysis for diluent and mobile phase preparation; it can be used for intended purposes in drug substance and drug product.
