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The quinalphos residue analysis was conducted using an isocratic mobile phase of methanol.
Resultant mixtures were subjected to HPAEC-PAD using mobile phase of 100 mM NaOH with 10 mM sodium acetate.
The column oven was maintained at 40 °C with a 1 mL min−1 mobile phase of chloroform.
using a mobile phase of 60% acetonitrile and 40% water at a flow rate of 1.0 mL/min.
Using an aqueous mobile phase of medium ionic strength, the elution was substantially regular and the macromolecules were not aggregate.
The HPLC apparatus was operated with a mobile phase of 80% acetronitrile and 20% H2O at a flow rate of 1.0 mL/min (Ferreira et al. 1997).
Fraction Fr-3-2 were further purified by HPLC using the mobile phase of 33%CH3CN/H2OO to give compounds 8 (18 mg) and 6 (16.9 mg).
Mobile phase of chloroform-methanol (9‐1, v/v) was for the first development, and petroleum ether-ethyl acetate (10‐1, v/v) was for the second development.
The HPLC method included an isocratic mobile phase of 90% water and 10% methanol with a flow rate of 1.0 mL/min.
Fr-IV was purified by silica gel column using the mobile phase of CH3Cl/CH3OH (3:1, v/v) to afford compound 17 (162 mg).
Simultaneous determination of cetirizine and aceclofenac was achieved by using mobile phase of acetonitrile and heptane sulphonic acid (Padmavathi and Niranjan [2011]).
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