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The wide range for linearity, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of CPT than the pharmacopoeial methods.
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As shown in Figure 6B, retentions of TALA decreased with the increase of concentration of 4-AP in the mobile phase, which indicated that TALA at least has one same binding site with 4-AP.
Isocratic performance strongly depends on the amount of gel porosity of the scaffold, which can be changed by varying the percentage of organic modifier in the mobile phase and indicates the adjustable chromatographic nature of porous polymer monoliths.
Data from the analysis phase indicated that discussion forums and text messaging with mobiles could be used as students were already using these tools.
The derivative product was separated with the gradient elution with the mixture of the mobile phase A+B as indicated with the volume proportion of the mobile phase B: 4 minutes from 0.5% to 26%, 4 minutes at 26%, 7 minutes from 26% to 100%, and finally another 7 minutes at 100%.
This monolithic column (referred to as naphthyl methacrylate monolithic column or NMM column) showed high mechanical stability at relatively high mobile phase flow velocity indicating that the column has excellent hydrodynamic characteristics.
The low values of % RSD obtained after introducing small but deliberate changes in mobile phase composition and wavelength indicated robustness of the methods (Tables 4(a)– 4(c)) at 3 different concentration levels.
The modified stationary phase with SDS monomers is negatively charged and the studied analytes are positively charged at the mobile phase pH (3.5) which indicates a strong electrostatic attraction to the stationary phase.
As demonstrated in Table 1, the theoretical plates with the mixture solution of methanol or acetonitrile with water as a mobile phase were below 1000 which indicated poor column chromatography separation power.
The column used was a Waters Symmetry C18 (packing: 3.5 μm) 4.6×150 mm with an isocratic mobile phase consisting of MeOH/H2O (as indicated) at a flow rate of 1.4 mL min−1.
The column used was either a Waters "Symmetry" C18 (packing: 3.5 μm, 4.6×150 mm) or a Waters "Sunfire" C18 (packing: 3.5 μm, 4.6×150 mm) with an isocratic mobile phase consisting of CH3CN/H2O (as indicated) with a flow rate of 1 mL min−1.
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