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Another practical advantage is a short time needed for column equilibration when a mobile phase gradient is used [29].
Column: Nucleodex β-PM; Injection: 20 μL; Mobile Phase: water and acetonitrile; mobile phase gradient (Table 1).
Optimum separation was achieved with a binary mobile phase gradient at a flow rate 0.5 mL/m.
Since the micro-RIG signal remains on-scale throughout the mobile phase gradient, one can apply a baseline correction procedure.
-Catechin and -epicatechin are separated by a reverse phase mechanism on a C18 column with an acidic acetonitrile-water mobile phase gradient.
A computated optimisation procedure, using the Polymer Chromatographic Model allowed us to design a step mobile phase gradient to improve resolution of homopolymer chromatographic separation.
We report a sensitive, highly reproducible, microfabricated refractive index gradient (micro-RIG) detector that performs well with mobile phase gradient elution LC.
Viscosity changes during the mobile phase gradient separation are found to shift the on-chip merge position of the detected concentration gradient (i.e., RIG), in a reproducible fashion.
The chromatographic analysis was performed using a mobile phase gradient with a flow rate of 1 mL min−1 and detection wavelength of 385/528 nm (λexc/λem).
The influence of steepness of the mobile phase gradient on separation of the oligonucleotides was evaluated as well as the reproducibility of HMMAA monolith preparation.
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A programmed mobile phase-gradient was used during a 10-min run: 0 min, 0.1% B; 6.8 min, 2% B; 6.9 min, 0.1% B; 10 min, 0.1% B. The concentration of the four nucleotides ATP, UTP, CTP and GTP was quantified at 270 nm absorbance.
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