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The optimum composition of mobile phase for the separation of the eight isoflavones was obtained.
An anionic micellar medium was added to the mobile phase for increasing the fluorescence intensity and peak efficiency.
In this study the 20 mM ammonium formate and acetonitrile in the 57 43 ratio were used as mobile phase for the analysis of valsartan.
The chromatographic separations were performed by RP-HPLC (250 × 4 mm, 5 μm) with isocratic elution using an acidic mobile phase for the three detection techniques.
The mobile phase was 5 mM NH4Ac in water (A) and acetonitrile isopropanol (1 1) as the mobile phase for gradient elution (45 100% B in 10 min at 0.4 mL min−1 and at 60 °C).
Elution of uncharged saccharides was performed at 0.7 ml min−1 using 50 mM sodium hydroxide and 20 mM sodium acetate in the mobile phase for 16 min followed by a sodium acetate gradient.
We were interested to develop a common mobile phase for all pyrethroids under study.
Sharp peaks were obtained with a presaturation of mobile phase for 20 min.
The mobile phase for the determination of MMC was water-methanol (65/35, v/v).
The mobile phase for the substance atrazine consisted of 68.5% water and 31.5% acetonitrile.
To make the method simpler, we used the same mobile phase for both the columns instead of different mobile phases.
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