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While the present implementation is a single sampling device demonstrating proof of principle, we intend to further develop EF-LESA to allow continuous mobile phase flow through the liquid tip, for online MS operation.
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The mobile phase flows through the packed bed or column.
Because of the transecting gigapores, a portion of the mobile phase flows through the pores in addition to fluid flow in the interstitial spaces between the particles in a packed-bed column.
The mobile phase flowed through an in-line Degassex DG4400 degasser (Phenomenex, Torrance, CA).
In the procedures developed by Wise and Saravanabhavan, the elution range of specific groups of analytes was determined by the volume and kind of the mobile phase flowing through the chromatographic column.
The mobile phase flow rate was 1 mL/min.
Mobile phase flow rate was maintained at 1 ml/min.
The mobile phase flow rate was 0.3 ml min 1.
The mobile phase flow rate was 100 µL min−1.
and a mobile phase flow rate of 100 μL/min.
The mobile phase flow rate was 1.0 ml min-1.
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