Modifications in mobile phase concentration, mobile phase volume, mobile phase saturation time, and temperature were examined to check the robustness.
In Supercritical Fluid Chromatography (SFC), the key chromatographic parameters of any compound, its retention and efficiency, are known to strongly depend on the density of the mobile phase.
The adsorption amount of Z-ligustilide from SC-CO2 on C18-bonded silica decreased with the increasing density of the mobile phase as well as the increasing temperature.
In SFC, the key chromatographic parameters, the retention factors and the column efficiency, strongly depend on the density of the mobile phase.
In industry, selection of appropriate mobile and stationary phase conditions is often empirically determined through a time-intensive, costly process of testing numerous combinations of variables such as stationary phase packing density, ligand loading, and particle size; mobile phase ionic strength, hydrophobicity, and pH; and column length, diameter, and flow rate.
In a companion paper, we analyzed the role of density in controlling the physical properties of the mobile phase, which in turn controls solute retentions and column efficiencies.
The pressure, temperature and density drops along SFC columns eluted with a CO2/methanol mobile phase were measured and compared with theoretical values.
The nature of this density dependence is explored with reference to the key physical properties of the mobile phase, its viscosity, diffusivity and solubility.
Two mobile phases were used: mobile phase (A) 0.05% (v/v) TFA and mobile phase (B) 100% acetonitrile.
