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The mobile phase contained 0.15 M sodium dihydrogen phosphate, 0.1 mM EDTA, 0.5 mM sodium octanesulfonic acid, 10 12% methanol (v/v), and 5 mM lithium chloride.
Each liter of mobile phase contained 100 mL methanol, 900 mL water, 6 g sodium acetate, 40 mg ethylenediaminetetraacetic acid (EDTA), 4 mg sodium dodecyl sulfate (SDS), and glacial acetic acid (to pH 4.0).
The mobile phase contained 20% acetonitrile, 20% methanol, 60% water and 0.1% phosphoric acid.
The mobile phase contained 280 μM ascorbic acid.
The mobile phase contained deionized water, formic acid (A; 99 1, v/v), and methanol (B).
The mobile phase contained acetonitrile (solvent A) and ammonium formate buffer (solvent B) adjusted to pH 6.8.
Similar(41)
Mobile phase containing 75% methanol and LiChrospher® 100 RP-18, 150 mm column were finally chosen.
The best separation was obtained with a mobile phase containing 50 mM phosphate, pH 5.5 and 6% methanol.
Chromatogrpahic separation by RPLC was achieved using a reversed-phase column and a water/acetonitrile mobile phase, containing 0.05% formic acid and using a gradient system.
Following termination of fermentation and extract preparation using above mentioned protocol, TLC profiles of the both the extracts were compared in mobile phase containing 5% MeOH in DCM.
A C8 chromatographic column (PerkinElmer, America) was used with the mobile phase containing 1 mM tetrabutylammonium hydroxide, 0.05 mM dipotassium EDTA, and 0.05% methanol (pH 6.8).
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