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The condition, however, that the composition of all components of the mobile phase must total 100% presents a problem when trying to mathematically represent ranges of each mobile phase constituent of a ternary or quaternary system.
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The effect of mobile phase constituents such as pH, type of buffer and buffer concentration on the surface chemistry properties on C18PZM are elucidated.
The full factorial design demonstrated that for all three plant references a C8 column (250 mm×4.6 mm; 5 µm particle size) is best suited; methanol and an ammonium formate buffer (pH 3) were found to be the best constituents for the mobile phase.
MeCN and 0.2%HAc were used as the mobile phase to improve the retention behavior of the constituents on the HPLC column.
Normal-phase chromatography involves a polar stationary phase and a less polar mobile phase.
In addition, phase constituent after hydrogenation is analyzed.
Two mobile phases were used: mobile phase (A) 0.05% (v/v) TFA and mobile phase (B) 100% acetonitrile.
Modifications in mobile phase concentration, mobile phase volume, mobile phase saturation time, and temperature were examined to check the robustness.
The mobile phase used was pure acetonitrile.
The mobile phase was acetonitrile (65%).
Water was used as the mobile phase.
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