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A mobile phase consisting of water (A) and acetonitrile (B) (each containing 0.1% formic acid) was used.
The solution was transferred to 5 mL of mobile phase consisting of acetonitrile and deionized water (50 50, v/v).
This solution was transferred to 5 ml of mobile phase consisting of deionized water and acetonitrile (50 50, v/v).
DI water and acetonitrile were used as the mobile phase consisting of 0.1%% (v/v) formic acid.
The trapped fluorescent derivative was back-flush eluted to fluorescence detector with an environmentally friendly mobile phase consisting of phosphate buffer and ethanol.
The mobile phase consisting of acetonitrile: 0.1% formic acid (84:16), was delivered at a flow rate of 0.6 mL/min.
An InertSustain RP-C18 column was used with the mobile phase consisting of methanol and 0.2% acetic acid as gradient elution at the detection wavelength of 332 nm.
In this method, a satisfactory separation was obtained with a mobile phase consisting of Acetonitrile: 0.1% (v/v) phosphoric acid in water (40: 60, v/v).
The previous experiment was performed using a mobile phase consisting of acetonitrile and phosphate buffer (pH 3) at a ratio of 60 40, v/v.
The standards and samples were resolved using a gradient mobile phase consisting of 5 mM ammonium acetate and acetonitrile; the flow rate was 0.5 ml/min.
The phenols were separated with a mobile phase consisting of 1% (v/v) formic acid (mobile phase A) and acetonitril (mobile phase B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com