Approximately 500 milligrams of each sample was extracted into methanol, filtered, diluted 1 20, and analyzed by HPLC, using a mobile phase consisting of 0.1% formic acid in water and 0.1% formic acid in methanol.
Chromatographic separation was achieved on a BEH-C18 column with a mobile phase consisting of 5 mM formic acid in water/acetonitrile (90 10, v/v) and methanol.
After preliminary studies, the reverse phase chromatographic system was selected using a cyano column and a mobile phase consisting of acetonitrile and phosphate buffer.
The separation process could be accelerated while also improving peak shape through the use of higher temperatures and a ternary mobile phase consisting of acetonitrile, tetrahydrofuran, and water.
GFX and MK in plasma samples were separated using a mobile phase consisting of a mixture of ethyl acetate:methanol:25% ammonia, (8 4.5 3, v/v/v).
A mobile phase consisting of water (A) and acetonitrile (B) (each containing 0.1% formic acid) was used.
The solution was transferred to 5 mL of mobile phase consisting of acetonitrile and deionized water (50 50, v/v).
This solution was transferred to 5 ml of mobile phase consisting of deionized water and acetonitrile (50 50, v/v).
DI water and acetonitrile were used as the mobile phase consisting of 0.1%% (v/v) formic acid.
The trapped fluorescent derivative was back-flush eluted to fluorescence detector with an environmentally friendly mobile phase consisting of phosphate buffer and ethanol.
The mobile phase consisting of acetonitrile: 0.1% formic acid (84:16), was delivered at a flow rate of 0.6 mL/min.
