Sentence examples for mobile phase comprising of from inspiring English sources
The peptides were separated using the mobile phase comprising of solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) in a gradient elution mode with a total run time of 100 min.
1.7 μm).The best peak resolution along with high sensitivity was achieved with an isocratic elution by a mobile phase comprising of acetonitrile: 10 mM ammonium acetate (60 40) at a flow-rate of 0.3 mL/min.
A mobile phase comprising of aqueous formic acid (0.1 %, v/v) (solvent system A) and acetonitrile (solvent system B) was employed with a gradient elution of 40 80 % B at 0 to 5 min, 80 85 % B at 5 to 10 min, 85 90 % B at 10 to 12 min, 90 95 % B at 12 15 min, 95%% B at 15 20 min.
Phosphocholine levels were analysed by high performance liquid chromatography (HPLC) with radiochemical detection using a μBondapak C18 column (7.8×300 mm, 10 μm; Waters, Watford, UK) and a mobile phase comprising of 1.5 m M K2HPO4 and 5 m M tetrabutylammonium hydrogen sulphate, pH 7.0.
The chromatographic condition using Nucleosil C18 column (250 × 4.6 mm internal diameter × 5 μm particles size) (Macherey Nagel, Germany) was maintained at 25 °C and the injection sample (20 μL) was eluted with an isocratic mobile phase comprising of methanol: tetrahydrofuran: water (0.1 % H3PO4) mixture in the volume ratio 55: 55 40.
The mobile phase comprised of acetonitrile/water with a gradient system as follows: 0 min.
Separation was achieved using a Phenomenex® Luna 5 μm (C18) column and a mobile phase comprised of phosphate buffer (adjusted to pH 3.0): acetonitrile in a ratio of 70 30 (v/v).
High performance liquid chromatography (HPLC) separation was conducted on an YMC ODS-AQ C(18) column with a flow rate of 0.3 mL/min using a mobile phase comprised of 5 mM ammonium acetate buffer/0.03% formic acid in the solvent mixture of methanol/acetonitrile/water (20 20 60, v/v/v).
The mobile phase comprised of A: methanol-10 mM KH2PO4-triethylamine in a ratio of 96:6:0.01 (v/v/v) and B: methanol.
The mobile phase comprised of acetonitrile (40%) and water containing 0.1% w/v triethylamine with pH adjusted to 3 with ortho-phosphoric acid (60%).
The HPLC mobile phase comprised of acetonitrile buffer (20∶80, v/v, buffer 50 mM KH2PO4, pH of 6.8 adjusted with KOH).
