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The sample was subjected to an elution gradient with a mobile phase comprising 35 85% (v/v) acetonitrile for 25 min followed by 85 100% (v/v) acetonitrile for 5 min.
A gradient elution with a mobile phase comprising 0.1% formic acid in water (A) and methanol (B) was used as follows: 0 min, 60% B; 16 min, 60% B; 16.1 min, 100% B; 20 min, 100% B; 20.1 min, 60% B; and 25 min, 60% B. The detector wavelengths were set at 270 nm.
One work [18] describes simultaneous determination of FEX and its two impurities A and B using C8 column as stationary phase and a mobile phase comprising 1% triethylamine phosphate (pH 3.7), acetonitrile and methanol in the ratio 60 20 20 (v/v/v).
The best resolution of peaks were achieved with an isocratic elution by a mobile phase comprising acetonitrile:water:formic acid (65 35:0.1%, v/v/v) at a flow-rate of 0.2 mL/min, on Acquity UPLC BEH™ C18 column (50 × 2.1 mm, i.d. 1.7 μm).
Because the pKa of oseltamivir is 7.75 (9 ), a mobile phase comprising a pH 10 (2 U above the pKa) bicarbonate buffer was chosen.
A mobile phase comprising 20% acetonitrile (Fisher Scientific, Leicestershire, UK) in 10 mM KH2PO4 was used at a flow rate of 1 mL·min-1.
Similar(39)
The mobile phase comprised of acetonitrile/water with a gradient system as follows: 0 min.
The final method uses a Kromasil column and the mobile phase comprises dioxane, water and ammonium formate solution pH 7.7.
The mobile phase comprises methanol, water, acetonitrile (ACN) and acetic acid (47:42:10:1, v/v/v/v) at a flow rate of 1.0 ml/min.
Separation was achieved using a Phenomenex® Luna 5 μm (C18) column and a mobile phase comprised of phosphate buffer (adjusted to pH 3.0): acetonitrile in a ratio of 70 30 (v/v).
High performance liquid chromatography (HPLC) separation was conducted on an YMC ODS-AQ C(18) column with a flow rate of 0.3 mL/min using a mobile phase comprised of 5 mM ammonium acetate buffer/0.03% formic acid in the solvent mixture of methanol/acetonitrile/water (20 20 60, v/v/v).
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