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Different gradient and isocratic mobile phase compositions were investigated.
The mobile phase was optimized by varying mobile phase compositions and flow rate.
Thereby two different mobile phase compositions are established in sections 1 and 2, and sections 3 and 4, respectively.
To different mobile phase compositions corresponds a different retention behavior of the solutes, i.e. different adsorption isotherms.
The designed mobile phase provided a significantly (p < 0.05) greater OTA peak area compared to other mobile phase compositions used as references.
The aim of the present study was to investigate the influence of different mobile phase compositions on the quantification (as measured by the peak area) of OTA.
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A method is described here, based on spherical coordinate representations, that adheres to the constraints of the mobile phase composition and allows experimental designs, such as central composite and factorial designs, to be applied to the simultaneous optimization of the mobile phase composition.
An experimental design was used for the optimization of three variables: mobile phase composition, flow rate and column temperature through a central composite design.
Four variables, including mobile phase composition and oven temperature, were analyzed through a central composite design exploring their contribution to analyte separation.
TLC results varie depending on mobile phase composition.
The LC mobile phase composition and flow rate were the same as the above-mentioned.
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