Sentence examples for mobile phase composition of from inspiring English sources

Results from thirteen mobile phase compositions showed that the mobile phase composition of ACN/NaAcetate (5 mM /MeOH/Acetic acid (pH 3); (30:40:30; v/v/v) resulted in the highest quantification value for OTA.

Rapid chromatographic separation was achieved in the mobile phase composition of 5-mM aqueous ammonium acetate and acetonitrile (v/v, 70 30) with a flow rate of 0.20 ml/min.

With the aim of achieving a good analytical performance, the variables that affected the separation were studied, using a mobile phase composition of 60 40 (v/v) acetonitrile/water containing ammonium acetate (1 mM, pH 6.5), a separation voltage of 25 kV and a temperature of 25 °C.

Table 2 shows that the mobile phase composition of 25 mmol/L phosphate: acetonitrile (35:65, v/v) at pH 4.0 achieved more than sufficient separation with a resolution of 8.2 and short retention times of both AML and FSM.

Optimum conditions: C18 monolithic column (4.6 × 50 mm), a mobile phase composition of 75 mmol/L phosphate:acetonitrile (70 30, v/v) at pH 3.0, flow rate 30 μL/s, sample volume 40 μL, UV detection at 270 nm for HTZ and 290 nm for PRP.

In the design of the gradient mode, we chose a mobile phase composition of 75% methanol in the first 5 minutes of the run to allow sufficient peak resolution of the amide and the ester peaks, and subsequently a gradient with increasing methanol content up to 95% was set over 10 minutes followed by holding for another ten minutes (Table 1).

The impact of the mobile phase composition on the peptide saturation capacity was also investigated.

In preliminary experimental investigations the effect of the mobile phase composition on the specific distribution equilibria was quantified.

The influence of the mobile phase composition on the adsorption equilibria in non-linear reversed-phase chromatography has been investigated.

The investigations in this study provide sound understanding not only on how to manipulate the separation selectivity through selection of appropriate mobile phase composition on the currently prepared columns but also on how to design next-generation columns with desired properties and functions.

The aim of the present study was to investigate the influence of different mobile phase compositions on the quantification (as measured by the peak area) of OTA.