Sentence examples for mobile phase component from inspiring English sources

Before HPLC/MS analysis, the IS was added to the final extract and the solution was made up to a volume of 10 mL with ammonium formate buffer (mobile phase component).

Formate buffer solution (mobile phase component, pH 4.5) was prepared by dissolving of 1.5 mL (20 mmol L−1) of formic acid in 2 L of water and adjusting the pH to 4.5 with aqueous ammonia solution.

Reconstitution was performed by first dissolving in methanol (ultrasonication) and then dilution with LC mobile phase component A. The LC MS MS method was validated in three separate studies, one using the API2000 with injection of 20 mg matrix equivalent and the other two using the API3000 with injection of 2 mg matrix equivalent.

Ruggedness of the method was determined by using mobile phase components from two different manufactures.

All the other mobile phase components were held constant as described above.

Aqueous mobile phase components and column temperature were optimized through Design of experiments (DoE) for a fixed gradient program.

The consequences of accounting for the adsorption of the mobile phase components themselves are investigated for the experimentally common situation of a linear inlet gradient.

Hence, the ratio of the mobile phase components n-Hexane/alcohol/diethylamine was fixed at 85:15:0.01 (v/v/v) and the effect of different alcohols on selectivity was studied (Fig. 3).

A Box-Behnken experimental design was used to study different experimental variables of the method, namely the ratio of the mobile phase components and the gradient time, and their influence in responses such as the retention factor, tailing factor, and theoretical plates for dapivirine and the IS, as well as the peak resolution between both compounds.

Amine and phosphate groups are polarized and interact with mobile phase components and solutes via polar interactions.

The HPLC mobile phase components were prepared by mixing methanol with buffer solution and acetone (component A, 69+24+7; component B, 11+82+7  v/ v/ v).