Sentence examples for mobile phase chloroform from inspiring English sources

The temperature of the columns was maintained at 40 °C, and the flow rate of the mobile phase (chloroform) was 1 mL min-1.

Calibration curve for glycyrrhetic acid is shown in (Additional file 1: Figure S1). Figure 1 HPTLC Chromatogram of standard glycyrrhetic acid (1000 ng spot -1 ): peak 1 ( R f = 0.42 ± 0.03), mobile phase; chloroform: methanol: formic acid (9 0.9 0.1).

15 mL of mobile phase (chloroform: methanol, 14 1 v/v) was used for linear ascending development and chromatogram was allowed to move to a distance of 8 cm, in twin trough glass chamber (CAMAG).

A constant sample application rate of 0.1 μL/s was employed and the space between the two bands was 9.1 mm. 10 mL of mobile phase (chloroform: methanol: formic acid, 9 1 0.1, v/v) was used for linear ascending development and chromatogram was allowed to move to a distance of 80 mm, in twin trough glass chamber (CAMAG).

Subsequently, glucocorticoids were extracted with dichloromethane, the organic phase evaporated, extracts dissolved in ethanol and separated by thin layer chromatography (on TLC aluminium sheets, 20×20 cm, Silica gel 60 F254, Merck KGaA, Darmstadt, Germany, mobile phase; chloroform and ethanol (92∶8)).

Sample solution was applied on the TLC plate and developed with mobile phase chloroform: ethyl acetate: formic acid (2.5 2 0.8, v/v/v).

Organic extracts were spotted on Silica Gel 60 HPTLC plates (Merck), pre-developed in chloroform-methanol (1∶1, v/v) and separated sequentially by two mobile phases: chloroform-ethanol-triethylamine-water (30∶35∶35∶8, v/v/v/v) and hexane-ether (100∶4.5, v/v).

Separation of individual lipid classes of polar lipids by normal phase (NP -HPLC was carried out using a Phenomenex Luna 3 μm-silica column (internal diameter 150 × 2.0 mm) with the following conditions : mobile phase A (chloroform: methanol:ammonium hydroxide, 89.5 10 0.5) and mobile phase B (chloroform:methanol:ammonium hydroxide:water, 55 39 0.5 5.5).

Fractions DIC to DIC were collected with mobile phase hexane: chloroform; 50 : 50 and fractions DIC to DIC were collected with mobile phase pure chloroform and were pooled on the basis of TLC.

Silica gel served as the granular solid, and Martin and Synge pictured the gel as composed of water tightly bonded to the crystals of silica; the mobile phase was chloroform.

The European Pharmacopoeia describes a liquid chromatography (LC) method for the quantification of sulindac, using a quaternary mobile phase including chloroform and with a rather long run time.