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Chloroform was used as the mobile phase at a flow rate of 1.0 ml min−1.
Acetonitrile was used as a mobile phase at a flow rate of 1.0 mL/min.
Tetrahydrofuran was used as the mobile phase, at a flow rate of 1 mL/min.
Figure 5 Plot of HETP of glycine and ammonium chloride versus the interstitial velocity of mobile phase at 50°C. Figure 6 Plot of HETP of glycine and ammonium chloride versus the interstitial velocity of mobile phase at 60°C.
The syringe was filled again with 3500 μL of the mobile phase at a flow rate of 150 μL/s.
Water was used as the mobile phase at a fixed flow rate of 0.6 ml/min, with isocratic elution.
H2SO4 (0.014N) with 12% CH3CN was used as the mobile phase at a flow rate of 0.7 mL/min.
The HPLC methods used were acetonitrile (A) and phosphoric acid (0.01 M) (B) as mobile phase at 6.0 mL/min.
Sulfuric acid solution (5 mM) was used as the mobile phase at a constant flow rate of 0.6 mL/min.
Sulfuric acid 0.01 mol/L was used as mobile phase at flow rate 0.5 mL/min (Liu et al. 2014a).
Separation was performed at 80°C, with H2O as mobile phase at a flow rate of 0.6 mL/min.
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