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The effects that give rise to pseudo-non-linear Van't Hoff plots, e.g. a temperature irregularity inside the chamber or heat evolving during solvent adsorption near the migrating front of the mobile phase are minimized.
However, the retention factors in the HILIC range of the mobile phase are almost independent of the temperature.
The resolution and peak asymmetries of the flavonoids and isoflavones using different organic acids in the mobile phase are shown in Fig. 1a.
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The mobile phase was purchased from Eicom and delivered at a rate of 0.33 mL/min.
The mobile phase was degassed by sonication prior to use.
The mobile phase was 0.05% trifluoroacetic acid in water.
In this case, the mobile phase is entirely nonselective.
The mobile phase was pumped at a flow rate of 1.8 ml/min.
The flow rate of mobile phase was set at 0.6 mL min−1.
The mobile phase was 30% methanol containing 0.05% TFA (LC-MS grade).
The mobile phase was 10 mM H3PO4-methanol (45:55, v/v).
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