Sentence examples for mobile phase 1 from inspiring English sources

The mobile phase 1 used for elution of paracetamol, caffeine and salicylic acid (internal standard) was acetonitrile/water (10 90, v/v, the water part of pH 3.5 adjusted with acetic acid), flow rate was 0.9 mL min−1 (volume 3.0 mL of mobile phase per analysis).

Chromatography column: ZORBAX Eclipse XDB-C18 4.6 × 150 mm, mobile phase: 1% HAc-MeOH (1 1, v/v); mobile phase velocity, 0.8 ml/min; column temperature, 30°C; sample volume, 20 μl; wavelength of ultraviolet detector, 277 nm.

After heating to 97°C for 30 min, the kit was neutralised with 1 M HCl and conversion to [99mTc(CO)3]+ was verified by thin-layer chromatography (TLC; glass-backed silica gel 60, Merck, Darmstadt, Germany; mobile phase: 1% HCl in methanol).

The HPLC conditions were as follows: chromatographic column, Shim-PackVP-ODS (250 × 4.6 mm) (Shimadzu, Kyoto, Japan); mobile phase, 1 mL L−1 trifluoroacetic acid (A) and methanol (B); column temperature, 30 °C; flow rate, 1.0 mL min−1; injection volume, 20 μL.

To each mobile phase, 1 mM AMF and 0.53 mM formic acid (FA) was added.

The mobile phase (1 mL/min) was 22 mmol/L NaOH.

The digested peptides were resuspended in mobile phase (0.1% formic acid in water) for LC MS analysis.

The optimum conditions were 40% of ethanol as mobile phase, 25 cm of bed height with the flow rate of mobile phase of 2.5 ml/min.

XBridge HILIC column with isocratic elution using mobile phase 10 mM K2HPO4 pH 7.2 acetonitrile (26:74; v/v) was employed.

The optimum conditions of separation (optimum values of significant factors) determined with the aid of central composite design were: (1) mobile phase: acetonitrile/H2O (67.5/32.5, v/v), (2) column temperature 40 °C and (3) mobile phase flow rate 0.9 ml/min.

The elution profile was 40% mobile phase 1 60% mobile phase 2 in a linear gradient from 0 to 20 min.