Sentence examples for mobile gradient from inspiring English sources

The mobile gradient was as follows: 10% A, 90% B, 0 0.5 min; linear gradient to 100% B, 0.5 15 min; composition held at 100% B, 15 34 min; return to initial conditions, 34 35 min.

The same solvents were used but the mobile gradient was as follows: 10% A, 90% B, 0 0.5 min; linear gradient to 100% B, 0.5 15 min; composition held at 100% B, 15-40 min; return to initial conditions, 40 41 min.

For both LC-MS and HPLC analysis a Reprosil 100 C18, 3 μm, 150 mm × 3 mm analytical column (Screening Devices) was used and applied with the following mobile phase gradient profile: 0 5 min 5%acetonitrile/95%% water, 5 30 min gradient to 95%acetonitrile/5%% water, 30 40 min 95%acetonitrile/5%% water.

The mobile phase gradient eluted isocratically with 95%% ACN for 0.5 min followed by a gradient to 35 % ACN over 12 min.

Typical refractive index (RI) detectors for liquid chromatography (LC) are not well suited to application with mobile phase gradient elution, due to the difficulty in correcting for the detected baseline shift during the gradient.

Viscosity changes during the mobile phase gradient separation are found to shift the on-chip merge position of the detected concentration gradient (i.e., RIG), in a reproducible fashion.

However, this viscosity effect makes detection sensitivity vary throughout the mobile phase gradient, due to moving the optimized position of the probe beam in relation to the analyte concentration gradient being probed.

Since the micro-RIG signal remains on-scale throughout the mobile phase gradient, one can apply a baseline correction procedure.

A computated optimisation procedure, using the Polymer Chromatographic Model allowed us to design a step mobile phase gradient to improve resolution of homopolymer chromatographic separation.

The chromatographic analysis was performed using a mobile phase gradient with a flow rate of 1 mL min−1 and detection wavelength of 385/528 nm (λexc/λem).

The influence of steepness of the mobile phase gradient on separation of the oligonucleotides was evaluated as well as the reproducibility of HMMAA monolith preparation.