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Exact(12)
Treatment with actinomycin-D reduced both the mobile fraction and the Deff by approximately two-fold (Figure 2G, Table 1).
In such experiments, monomeric proteins that do not interact with any cellular components, such as EGFP [42], [43], should have a high mobile fraction and a diffusion coefficient consistent with their size and shape.
We assessed both recovery (mobile) fraction and t1/2, using unbleached puncta as controls.
This value was defined as the mobile fraction, and the stable fraction was defined as (1 − [mobile fraction]).
This pattern is more evident in the FRAP analysis of mitochondrial connectivity, which displays increased mobile fraction and decreased recovery time in Anxa6-expressing cells.
The y intercept value was taken as the size of the mobile fraction, and the stable fraction was then calculated as above.
Similar(48)
Transformations between the mobile fractions and Fe/Mn oxide-bound fraction occurred during the 6-week incubation in both soils.
Thus, despite Cd-content being very low, it appeared in the mobile fractions and may easily pass on to water.
Table 2 shows the mobile fractions and the halftimes (T1/2) of the neurons studied in this experiment.
Mobile fractions and recovery half-times (t1/2) were obtained for each recovery curve, and the means and standard deviations were calculated.
Nevertheless, we observed a remarkable differential recovery of Ezh2 in response to transcriptional stress that was accompanied by a faster diffusion rate of the mobile fraction of Ezh2 and a significantly increased immobile fraction, suggesting tighter chromatin binding of Ezh2 upon transcription inhibition.
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