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The mobile composition decrease to 0% A was done in 3 min and kept at 0% A for 7 min and returned to 100% A in 0.5 min with a final hold of 1.5 min.
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The mobile phase composition was adjusted to the composition at chromatographic elution from the HPLC column of the respective compound.
A method is described here, based on spherical coordinate representations, that adheres to the constraints of the mobile phase composition and allows experimental designs, such as central composite and factorial designs, to be applied to the simultaneous optimization of the mobile phase composition.
Four variables, including mobile phase composition and oven temperature, were analyzed through a central composite design exploring their contribution to analyte separation.
An experimental design was used for the optimization of three variables: mobile phase composition, flow rate and column temperature through a central composite design.
TLC results varie depending on mobile phase composition.
The LC mobile phase composition and flow rate were the same as the above-mentioned.
With regard to mobile phase composition, methanol/H2O system was better than acetonitrile/H2O system.
In the current study, the second mobile phase composition performed sufficient separation with acceptable peaks.
For the optimization of mobile phase composition, acetonitrile, primarily, shows better separation than methanol.
Before MS analysis, liquid chromatographic method with suitable mobile phase composition was developed.
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