Sentence examples for mn in buffer from inspiring English sources

For membrane-bound TGFβ, cells were labeled with biotinylated anti-TGFβ antibody (R&D, Minneapolis, MN) in buffer containing BSA followed by streptavidin-PE (BD, San Diego, CA).

For the flow cytometric adhesion assays, cells were tested for binding to 10 µg/ml of a mouse E-selectin/human Fc chimera (R & D Systems, Minneapolis, MN) in buffer containing 2mM CaCl2 because binding is calcium dependent, and with and without 2 mM EDTA (a calcium ion chelator), or with 10 mM EDTA alone as controls.

We demonstrated a palladium-based electrochemical sensor for CSV and demonstrated the determination of Mn in buffer and environmental water samples.

The molecules that passed through the filter were collected and then incubated with recombinant rat Nogo receptor/Fc chimera (R&D Systems, Minneapolis, MN) in selection buffer (PBS, pH 7.4, 3 mM MgCl2) for 30 mins.

96-well ELISA plates were coated with 1 ug/ml mouse VEGFR1/Fc and VEGFR2/Fc (R&D Systems, Minneapolis, MN) in sensitizing buffer (0.621 g NaHCO3 and 0.275 g Na2CO3 dissolved in 100 mL of ddH2O, pH 9.5) overnight at 4°C.

Membranes were first blocked for 1 h in TBS containing 0.01% Tween 20 (Sigma) and 5% skim milk, and then incubated overnight at 4°C with primary antibodies against human SHBG (SHBG mouse monoclonal 11F11 antibody, kindly provided by Dr. Geoffrey Hammond; and SHBG goat polyclonal AF2656 antibody, R&D Systems, Minneapolis, MN) in the same buffer.

The Pd WE sensor exhibits favorable response for Mn in pH 9.0, 0.1 M borate buffer with a detection limit of 334 nM (18.3 ppb).

Chromatin (equivalent to 1 μg DNA) was digested using 0.0014 U MNase in the Atlantis MN Digestion buffer.

The DPPC dispersions of 1000 ppm at 37 °C with the new method produced, at pulsating area conditions at 20 cycles per minute, low tension minima (γmin), lower than 10 mN/m, in buffers (1), (2), and (4).

Control samples consistent of purified CAIX protein in buffer with low, medium and high concentrations as included in the ELISA kit ("MN/CAIX ELISA controls").

Adding 10 ppm Mn to the buffer illustrates the reduction peaks for Mn in the 0 to 200 mV range.