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The treatment of BR for 60 days and single dose of EMS or MMS on day 61, resulted in significant (p <0.05) reduction in the frequency of chromosomal aberrations in comparison to EMS or MMS treatment alone, indicating a protective effect of BR.
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The average size of tumors was 164.3 ± 25.4 (SEM) mm (standard conditions) and 155.7 ± 29.8 (SEM) mm (EE conditions) on day 13.
IMP concentrations at 20 °C increased to 2.1 mM on day 2 (P < 0.05), remained steady between days 3 and 4 (P > 0.05), and decreased to 1.8 mM on day 5 (P < 0.05).
AMP concentration at 20 °C was 1.1 mM on day 1 and decreased to 0.59 mM on day 5 (P < 0.05).
For the BC membrane alone, ALP concentration went from 2.5 mM on day two to almost 5 mM on day four.
There was a positive trend with a decline of MM on day 7 - 0.248 ± 0.001.
The level of HxR increased after day 4 to a maximum of 0.82 mM on day 5. Hx increased after day 2 to a maximum of 0.64 mM on day 3, then decreased slowly to day 5 (Fig. 2c).
AdR was detected and increased after day 1 to a maximum of 0.52 mM on day 2, but decreased after day 3. HxR increased after day 1 to a maximum of 0.90 mM on day 3 and then decreased to day 5. Hx increased after day 2 to a maximum of 3.2 mM on day 4 and then decreased on day 5 (Fig. 2d).
The number of large follicles (≥5 mm) on day 19 was reduced in feed-restricted gilts (16.9 versus 20.6, P<0.05).
Bacterial isolate 5 slowly degraded AMP to a minimum of 1.1 mM on day 5. IMP was not detected, but AdR was present at 0.19 mM on day 3.
This medium was further supplemented with sucrose (1%) and ammonium citrate (50 mg/l) on day 10 and sucrose (1%) and phenylalanine (0.1 mM) on day 20 (i.e., biomass growth medium).
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