Stock solution of etoposide (Teva, Israel), at 34 mM, was stored at room temperature.
The next 3.5 mm was stored at –20°C pending a mechanical push-out test.
ATP is abundant in the cell cytoplasm (2 5 mM) and higher concentrations of ATP (up to 100 mM) are stored in synaptic vesicles of neurons.
Amino acids Amino acid stocks were prepared at 10 mM concentration or their maximum solubility (always >1 mM) and were stored at –20°C.
Aliquots of a 2 mM stock solution of cisplatin and oxaliplatin in 40 mM NaCl were stored in dark at -20°C, defrosted and diluted with cell culture medium to the desired concentration before use.
Then the sieved soil, less than of 2 mm size, were stored in acid-washed labeled plastic containers until further analysis.
The extracts were filtered by Whatman filter paper # 1 (150 mm) and were stored at -40°C until used.
Stock solutions of TAK-242 were dissolved in DMSO to a final concentration of 100 mM, and were stored at −80°C.
Protein extracts, prepared as in Hesketh et al. [ 18], dissolved in denaturing isoelectric focusing buffer UTCHAPS (7 M urea, 2 M thiourea, 4% (w/v) CHAPS, 50 mM DTT, 4 mM Pefabloc SC protease inhibitor, and 40 mM tris pH9.0), were stored frozen in aliquots at -80°C.
Cultures were centrifuged at 5,000 ×g for 20 minutes at 4°C. Cell pellets were washed twice with buffer A (100 mM Tris pH 7.5, 150 mM NaCl) and were stored at −80°C until used.
