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Replicate animals (5.9 ± 1.72 g and 33.9 ± 2.13 mm) were stocked randomly on individual plates, and four paste diets containing PE ratios ranging from 87.2 to 102.9 mg/kcal were offered once daily (1600 h).
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Solutions of P1 and P2 in optimized concentration range of 0.14 0.35 mM were prepared from stock solution made of using 0.5 M HCl.
The experimental disk abalones, of shell length 24.5 ± 0.5 mm, were cultured at three stocking densities, 700, 1300 and 1910 individuals/m2 bottom area, in triplicate.
A 20 mM main stock of curcumin was prepared in absolute ethanol and then substocks from 0.034 mM to 1 mM were prepared in sterile deionized water from the main stock.
Silver nitrate (AgNO3) at a final concentration of 0.5 mM was added from a higher stock of 200 mM to the cell filtrate and agitated at 100 rpm in dark at 25°C.
A concentrated dicamba stock (7 mM) was diluted in water.
The concentrated 2,4-D stock (1 mM) was made in DMSO and then diluted in water.
Briefly, an equal volume of the DTNB (Sigma) working stock (5 mM) was added to the filtrate obtained following the synthesis reaction.
Fura-2AM (acetoxymethyl) (Life Technologies) stock (1 mM) was prepared in 100% DMSO and stored at −20 °С.
BCECF-AM stock (5 mM) was purchased from Beyotime (Nantong, Jiangsu, China) and 10 uM final concentration was applied to the cells.
Although the pigmentation of P1-mm is highly unstable, stocks that exhibit different levels of pigmentation including infrequent self-red revertant alleles that resembled the phenotype of P1-rr have been maintained [7], [9], [10], [11].
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