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Liquid silicate droplets (~2 mm) were maintained stable in levitation using a nozzle with a 0.8 mm bore and an opening angle of 60°.
Paired adjacent explants of approximately 6 × 6 mm were maintained in culture in 4 ml of Dulbecco's modeified Eagle's medium/HAMF12 1:1 (Invitrogen, Paisley, UK) in the presence or in the absence of 10% FBS (Invitrogen) and antibiotics/antimycotics (Invitrogen) in individual 33 mm bacteriological Petri dishes (BD Falcon™, BD Biosciences, Le Pont De Claix, France).
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A consistent slump in the range 75 100 mm was maintained for all mixtures.
A gap of 0.025 0.05 mm is maintained constantly between the wire and work-piece.
A clear cover at the top and bottom of the beam was 20 mm, whereas a clear cover of 15 mm was maintained on the beam's vertical sides.
It is advised that a bone bridge of approximately 10 mm be maintained on the lateral side and that the lateral extent of the osteotomy be closer to the lateral cortex than to the lateral joint line.
Euglycemia (~7.0 mM) was maintained during the insulin clamps.
A constant ionic strength of 100 mM was maintained in all samples using NaCl.
The flow rate was 1.0 ml min−1, the solvent was 20% (v/v) methanol/20 mM sodium acetate buffer (pH 5.0), and the ODS column (C18, 4.6 × 250 mm) was maintained at 35°C.
During the insulin infusion, euglycemia at a blood glucose concentration of ~5.5 mM was maintained by variable rate infusion of 20% dextrose solution (Baxter, Deerfield, IL) which was enriched (2.5%) with [6,6- H2glucose.
One can also rule out that tactile stimuli caused the perceptions described by subjects since the acupuncture points were localized without any skin contact, and during treatment, a distance between skin and laser of 5 mm was maintained.
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