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In a typical binding experiment, the cell was filled with 40 μM cellobiohydrolase, and pure XOS (1.25 mM) or pools of mixed oligosaccharides (low XOS 5 g/L about 8.4 mM or high XOS 5 g/L about 4.4 mM) were injected according to the following protocol: initial equilibration for 16 minutes, then nine injections of 1 μl after 150 seconds, followed by fifteen injections of 2 μl.
We found that possible smallest particles with average diameter 1.3 ± 0.3 nm were produced when aqueous solutions of H2PtCl6 (4 mM) and NaBH4 (40 mM) containing PVP (160 mM) were injected into the micromixer placed in an icebath at a flow rate of 200 mL/h.
To archive the maximal knock-down effect, 1 nl of serially-diluted MOs (purchased from Gene Tools) at the concentrations of 1, 0.5, 0.25 and 0.1 mM were injected into yolks at the 1∼2-cell stage.
In a typical experiment, 1.5 or 2 µl aliquots of SRC-1 NR2 peptide (676-CPSSHSSLTERHKILHRLLQEGSPS-700) at 1.3 mM were injected at 0.5 µl.s−1 into a 20-50 µM RAR, RXR or RAR/RXR complexes solution (200 µl sample cell).
Three weeks later, tumors with a diameter of about 5 mm were injected with 100 µL Ad buffer (10 mmol/L Tris-HCl pH 8.0, 2 mmol/L MgCl2, and 4% sucrose) containing 1×109 PFU of CNHK500 [7] or Ad buffer alone.
Ringer solution (control), octopaminergic (mianserine 0.33 µM or 3.3 mM, epinastine 4 mM) or dopaminergic receptor antagonists (fluphenazine 0.19 µM or 1.9 mM, flupentixol 0.2 µM or 2 mM) were injected (10×20 nl in all cases) into the brain through the median ocellar tract 30 min before conditioning.
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The stable ATP analogue α,β-methylene adenosine 5′-triphosphate (α,β-meATP, 10 mM) was injected into the ipsilateral temporal muscle, 30 min later followed by injection of local anaesthetics (lidocaine, 2%%) into the ipsilateral neck muscles and/or the temporal muscle.
KA (20 mM) was injected unilaterally by mean of a cannula connected to a precision pump into the intra-hippocampal region.
Micro channels having 186-micron pitch distance and 15-micron height on a substrate of size 15×5.88×0.49 mm is injected and subsequently, debound and sintered successfully.
For four weeks, a stream of groundwater with added As V) (6.7 μM) and bromide (1.6 mM), was injected in order to observe the reduction of As V) to As III).
After reacting for 20 min, 1 mL of dilute HNO3 (5 mM) was injected into the above solution to etch the Cu2O cores.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com