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Specimens with an approximate volume of 600 mm were fixed with Karnovsky fixative for at least two hours, washed in 0.15 M phosphate buffer for 10 min, transferred into osmium tetroxide solution and incubated for 2 h at 4°C.
Small pieces of 1 mm were fixed in the same fixative at 4°C for at least 24 h, post-fixed in 1% osmium tetroxide and dehydrated in acetones before embedding in Spurr resin.
The studs, with diameter of 10 mm, were fixed with epoxy resin.
Samples with a thickness of 2 to 3 mm were fixed with 2.5% (v/v) glutaraldehyde in 0.2 M phosphate buffer (pH 7.2) for 2 h.
Tobacco leaf thin sections (less than 0.5 mm) were fixed by vacuum infiltration with 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.4.
Specifically, all tissues (median tumor length: 7 mm) were fixed or snap-frozen within 15 to 30 minutes of excision.
Similar(40)
A plate distributor with 100 holes of diameter 3 mm was fixed in the bottom of the fluidized bed.
A column made of acrylic material with outer diameter of 140 mm was fixed at the center of tank to provide space for LED luminaire.
A custom unicondylar femoral component with a radius of 37 mm was fixed to the actuator arm of the MTS machine.
Groups of 12 sheep were formed and in each group a different osteotomy gap (0, 1 and 3 mm) was fixed using a locking compression plate (LCP) in combination with the DLS.
Next, d = 6.5 mm was fixed, and the effect of ET/ EM was studied.
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