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Inhibition zones (mm) were determined using an agar diffusion assay when target bacteria were used as indicator organisms.
The dimensions of a perfect taper based on 6726 CMM points with a point spacing of 0.3 mm were determined using a least-squares method.
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The percentage of sand (0.05 2.0 mm), silt (0.002 0.05 mm) and clay (<0.002 mm) fractions of the fine earth (<2 mm) was determined using the modified sedimentation hydrometer procedure (Bouyoucos 1962).
Tumour volume (V), in mm, was determined using the following equation: length × width/2 l × w/22).
Tumour volume (V), in mm, was determined using the following equation: V=length × width/2.
The numerical density of CA pyramidal neurons (neurons per mm) was determined using previously published methods (Dentremont et al., 1999).
Time to progression, defined as a function of time to tumour growth of greater than 400 mm, was determined using a Cox regression model.
Tumor volume (mm) was determined using the formula 0.5 × length × width, and the fold change in volume was calculated by dividing the obtained value by the tumor volume on the day before the treatment was started (day 0).
The growth-inhibitory effects upon MM cells were determined using a 3- 4, 5-dimethyl-2-thiazolyl -2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich).
Perfused and pulverized liver tissue from each animal was solubilized in lysis buffer (20 mM Na-Hepes, 0.1%(v/v) SDS, 150 mM NaCl, 1 mM EDTA, 1 mM NaVO3, 10 mM NaF, 1 mM EGTA, 1 mM PMSF), and protein concentrations were determined using the Bradford Protein Assay.
Radiation absorbed-dose estimations for human tissues following 64Cu-MM-302 administration were determined using OLINDA/EXM and are presented in Table 2.
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