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Phenolic compounds with concentrations ranging from 0.2 to 5 mM were administered to the cultures at 5 DAS unless otherwise specified.
For the efficacy experiment, 12 doses of D-WAT (1 mM) were administered s.c. into lower back (585 μg/injection/mouse) over a period of 4 weeks, after which animals were analyzed for a period of 8 more weeks.
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N-Methyl-d-aspartate (NMDA; 0, 10 10, 50, and 100 mM) was administered through guide cannulae aimed at the SCN at circadian times when light induces phase shifts.
All participants were in a fasting state when Konakion® MM was administered.
Ascorbic acid treatment (0.25 mM) was administered for 72 h (Sigma).
NS102 or Auranofin dissolved in dimethlysulfoxide (DMSO) at a concentration of 10 mM was administered (10 μl, intracerebroventricular) 30 min before ischemia.
Using a double-blind, randomized, crossover design, 23 individuals (9 males:14 females; age, 25 ± 2 years; body mass index, 22 ± 0.6 kg/m2; systolic BP/diastolic BP, 113 ± 3/70 ± 2 mm Hg) were administered 400-mg Rg3-KRG extract or 400-mg wheat bran control on two separate visits with a 7-day washout period.
When tumors reached 100 mm, mice were administered an i.p. injection of bortezomib (0.5 mg/kg body weight) twice weekly for three to four weeks.
The contrast agents were diluted in 0.9% saline to a final concentration of 60 mM (Gd-DTPA) or 7.0 mM (gadomelitol) and were administered in the tail vein in a bolus dose of 5.0 mL/kg.
or a solution of 100 mM Manganese Chloride were administered, either by direct feeding or microinjection, during larval, pupal, or adult phases of the life cycle.
To test the role of a single session of exercise on AICAR-increased food intake, AICAR (2 mM) or its vehicle were administered (i.c.v). to control and exercised animals.
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