CFT (150 × 164 × 3 mm) was adhered to the inside of a carbon plate (150 × 35 × 6 mm), and 8 carbon plates holding CFT were used to equip a cathodic working chamber (2.4-L working volume) as the cathodic working electrode and supporting material.
A small metal bar (height 2 mm) was adhered to the base of the reaching window to create a barrier.
To test this, we built the Fly Duplex, which we constructed by using three standard 25 mm × 75-mm glass microscope slides that were adhered between two 75 mm × 50 mm × 1-mm glass microscope slides using clear aquarium silicone sealant, making two compartments separated by one 1-mm thick glass slide.
Fly duplexes were constructed by using three standard 25 mm × 75-mm glass microscope slides (VWR (Radnor, PA): Item No. 48,300-025) that were adhered between two 75 mm × 50 mm × 1-mm glass microscope slides (Fisher: Item No. 12-550C).
Sections of each fibrous membrane (PCL, PCL/dex, PLLA, PLLA/dex) were adhered to 21 mm glass slides (VWR) using a medical silicon adhesive (Factor II).
Purified reaction centres were adhered to 2 mm diameter cleaned gold electrodes and inserted into a detergent-free buffer solution containing 20 μM cyt c and 30 µM UQ0 as mediators (see Section 2).
The sample pad, the pretreated conjugate pad, the nitrocellulose membrane, and the absorbent pad were adhered to a backing plate (300 mm × 60 mm) in the proper order.
Monocytes were adhered to plastic 60 mm culture dishes in serum-free DMEM.
Silver disk electrodes of 10 mm in diameter with bipolar leads (Duotrode; Morita Corp., Osaka, Japan) were adhered on both sides of the central part of the masseter muscle.
Whole Cell Patch Clamp DT40 cells were adhered to glass coverslips by a 10-min incubation in serum-free media and then stored in standard media (see above) until transferred to the extracellular solution (145 mM NaCl, 2.8 mM KCl, 10 mM CsCl, 1 mM CaCl2, 2 mM MgCl2, 10 mM glucose, 10 mM HEPES, pH 7.4).
