After thawing, DNAse I (20 μg ml−1) and MgSO4 (1 mM) were added and cells were lysed by sonication.
Different percentages of waste PET with maximum size of 2.36 mm were added to SMA mixtures.
The substrates dissolved in dimethyl sulfoxide at 10 mM were added to the samples to a final concentration of 0.1 mM.
LPS (10 ng/mL), IFN-γ (10 ng/mL) and magnesium (5 mM) were added in the medium separately or together as planned.
Then, a mixture solution of HCOONa (500 mM) and HAuCl4 (20 mM) were added to wrinkled PDMS substrates at room temperature for 1 h.
l-Carnitine doses of 0.5, 1 and 2 mM were added to the first portion, and glutamine was added at the same doses to the second portion.
In the third protocol, neurotrophic factors (NFs; namely BDNF, GDNF and NGF-β, all at 10 ng ml−1) and db-cAMP (0.5 mM) were added to the medium35, as basal cells upregulated several receptors (for example, NGFR, NPBWR1).
In the third protocol, NFs (BDNF, GDNF and NGF-β, all at 10 ng ml−1) and db-cAMP (0.5 mM) were added in the medium35, as basal cells upregulated several receptors for these NFs (for example, NGFR, NPBWR1).
For EDC-NHS chemistry, 100 μl of EDC (4 mM) and 100 μl of NHS (10 mM) were added to all the wells of the nanostructured 96-well polystyrene plates, which were incubated for 10 min.
Hundred-microliter GHS solutions (195.0 mM) were added to SR-MNPs.
In both of them bacterial biomass and Cd2+ (1 mM) were added.
