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Images were taken every 10 min and the number of apoptotic cells per mm was quantified using the CellPlayer 96-well Kinetic Caspase 3/7 reagent and the IncuCyte-FLR-Platform (Essen Biosciences).
Immunofluorescence (IF) for β-gal+, Pva +, and Sst + in the hippocampus (bregma levels −1.34 to −2.80 mm) was quantified using coronal cryosections (20 µm thick) from 4-week old animals.
The tumour area (mm) was quantified from videotape by digital image analysis.
The levator muscle function (mm) was quantified as follows: 10 15 mm: excellent; 8 mm: good; 5 7 mm: sufficient; <4 mm: poor.
The residual hole cross-sectional area (mm) was quantified at the top of the hole and at three different depths (0.5 mm, 1.0 mm and 1.5 mm) from the top of the hole using a polygon tool (CTAn software, Skyscan, Kontich, Belgium) to draw a 2D region of interest in the axial plane that circumscribed the non-mineralized hole edge (Additional file 1: Figure S1A).
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Biopores of two diameter classes (2 5 mm and >5 mm) were quantified per unit surface area by visual classification in 45 145 cm soil depth.
White matter lesions (≥5 mm) were quantified on T2-images according to the age-related white matter change score (ARWMC; Wahlund et al., 2001), which was used in myotonic dystrophy types 1 and 2 recently (Romeo et al., 2010).
Scales were labelled "unsure" (0 mm) and "sure" (100 mm) and the rating was quantified in mm.
Acute sensitivity to 1 mM aldicarb (Sigma) was quantified by measuring the time to paralysis onset in a population of worms [19], [38].
Number of microglia per mm of hippocampus was quantified using the Image J software (NIH).
Risk of abnormal cIMT (>0.6 mm) in patients was quantified by an odds ratio (OR) with a 95% confidence interval.
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