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Styrene-butadiene rubber (SBR) packed in a cylindrical mold of inner diameter 74.6 mm, was heated by one-dimensional, transient heat conduction from the mold.
A solution of silver nitrate (125 ml, 1 mM) was heated to 95°C, and a solution of trisodium citrate (7.75 mM) was added.
Aqueous chloroauric acid solution from 0.25 to 2.5 mM was heated to boiling and the four times molar amount of sodium citrate was added, followed by continuously heating for a certain period to get the ruby-red colloids.
A 500 mL amount of an aqueous solution of HAuCl4 ([Au3+] = 0.5 mM) was heated until heavy boiling.
A C18 reverse-phase column (3 μm microsorb, 100 mm × 4.6 mm) was heated to 40°C.
In brief, 50 mL of gold III) chloride trihydrate solution in bidistilled water (final concentration 1.14 mM) was heated in a clean Erlenmeyer flask under stirring until boiling.
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Samples treated with sample buffer and β-mercaptoethanol (2-ME) with a final concentration of 14.4 mM were heated at 100°C for 5 min before 15% SDS-PAGE.
A total of 100 stainless steel spheres (d = 3.2 mm) were heated in a crystallizing dish to 70 °C with a heat gun.
Cadmium oxide (CdO, 99.99%, Alfa Aesar, 0.0514 g, 0.4 mM) along with tetradecylphosphonic acid (TDPA, 98%, Alfa Aesar, 0.2232 g, 0.8 mM) and TOPO (3.7768 g, 9 mM) were heated with continuous stirring in a 125 mL glass flask.
0.2 mg/ml of the purified protein in 20 mM Tris-HCl pH 8.0 and 100 mM NaCl was heated from 10°C to 100°C.
First, 20 ml of 1 mM HAuCl4 was heated to a boil with constant stirring and with reflux.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com