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Thereafter, a rectangular scleral flap measuring 4 × 3 mm was dissected.
An area of approximately 10 30 mm was dissected from each patient and isolated in 300 μl of lysis buffer (Gentra Systems Inc., Minneapolis, MN, USA).
The NP was isolated after the outermost layer of the AF (about 0.5 mm) was dissected and discarded to prevent contamination with cells from surrounding ligaments.
After conjunctival incision at the 12 o´clock position, a superficial parabolic scleral flap measuring 5 × 5 mm was dissected and a smaller deep flap was created to allow access to Schlemm canal.
The septal coronary artery (4 6 mm) was dissected, cleared of adhering tissue and incubated with 20 μM Fluo-4 AM for 3 h at room temperature in the presence of 0.25% of the non-ionic detergent Pluronic F-127.
Five eyeballs in Group C were randomly selected and were used for the biomechanical test of the sclera by comparing with Groups A and B. One scleral strip (size, 2.0 mm × 6.0 mm) was dissected from the equatorial sclera of the superonasal quadrant.
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The portal vein (diameter, 5 10 mm), supra- and infra-hepatic inferior vena cava (IVC) (diameter, 12 15 mm), and common bile duct (diameter, 1.5 3.0 mm) were dissected out.
The distal parts of the soleus and gastrocnemius muscles (5-8 × 10 mm) were dissected out.
Thin ventricular trabeculae (diameter <0.6 mm) were dissected with the help of a stereoscopic microscope.
For the UV spectroscopic analysis, small blocks (approximately 1 × 1 × 0.5 mm) were dissected from the rind and pith samples.
Secretory-stage and transition-stage enamel samples, measuring 1.5 2 mm, were dissected apical to the white opaque zone.
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