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The durum wheat endosperm component of the meal was provided in the form of finely milled flour (<0.2 mm) to create a smooth porridge or coarsely milled macroparticles (∼2 mm) to create a coarse porridge.
Then, the CTVs were expanded 1 2 mm to create the planning target volumes (PTVs) in consideration of setup error and patient motion.
The CTV was uniformly expanded by 5 mm to create a planning target volume (PTV) to account for a set-up error including rotation.
The MZI air-gap mismatch was set to 8.4 mm to create a k-clock that supported an OCT imaging depth range of 4.2 mm.
Latrunculin A (Sigma, St Louis, MO, USA) was dissolved in DMSO to a concentration of 1 mM to create a stock.
In that boost plan, clinical target volume (CTV) was expanded with a three-dimensional margin of 3-4 mm to create a smaller PTV for planning.
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Samples were then overlayed with 1.6 ml 30% and 0.8 ml 5% sucrose (in 150 mM NaCl; 20 mM Tris/HCl pH 7.4; 0.1 mM EDTA) to create a sucrose gradient.
Iliac crest autografts were simulated by wooden blocks (20 × 30 mm) cut to an appropriate gap length, with a 1 mm overhang to create a "pressfit" situation that reflects the method used in the clinical setting.
Seedlings with primary roots 12 20 mm in length were transplanted into vermiculite mixed with pre-determined amounts of 1 mM CaSO4 to create high (-0.03 MPa) or low (-1.6 MPa) Ψw and grown under near-saturating humidity conditions to prevent further drying of the media.
Fujifilm's FinePix REAL 3D technology captures true 3D thanks to its use of twin 10 MegaPixel CCD sensors and dual FUJINON 3x optical zoom lenses (35-105mm) thareare spaced 75 mm apart to create realistic images that are similar to how human eyes see them.
Cells staining positive for TRAIL, αSMA and apoptosis (TUNEL) were counted as a proportion of the total cells in 0.1 mm plaque to create a percentage index.
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