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TRN targeting was achieved by guiding stereotrodes and optical fiber through a linear array (dimensions ∼1.1 x× 1.8 mm) secured to the bottom of the hyperdrive by cyanoacrylate.
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Catheter tubing was inserted approximately 7 mm into the vein and secured to the vein and surrounding tissue with sterile suture.
For this purpose, mice were anesthetized, and under sterile conditions a 26-gauge stainless steel cannula was implanted stereotaxically into the lateral ventricle using the bregma as a reference (0.5 mm; 1.1 mm lateral; depth 3 mm) and secured to the cranium using superglue.
We monitored 144 nest boxes with six different entrance sizes (20, 35, 55, 75, 95 and 115 mm diameter), secured to individual trees of three sizes (small 20 50 cm DBH, medium 51 80 cm and large >80 cm) situated in four different landscape contexts with varying degrees of modification (reserves, pasture, urban parklands and urban built-up areas).
A 4 mm titanium fixture is secured to the bone.
A potted #7 BFX femoral stem with 17 mm BFX head was secured to the actuator of the materials testing machine.
Each hemipelvis was mounted in a testing fixture and a #7 BFX femoral stem with a 17 mm BFX head was secured to the actuator of the materials testing machine to load the acetabular cup.
A 26-gauge cannula of polyethylene tubing (PE-10: inner diameter 0.28 mm, outer diameter 0.61 mm) was implanted into the lateral cerebral ventricle (1 mm caudal to Bregma, 2 mm lateral to the mid-sagittal sinus, and 4 mm ventral to dura) and secured to the skull with surgical glue.
A single loop of 3-0 prolene suture was passed through the midline of each construct at a distance of 5 mm from the cut edge and secured to a immovable cylindrical rod mounted on the material testing machine.
The two fixator plates were lowered down consecutively to a distance of about 5 mm above the surrounding muscle surface and secured to the pins.
Distal fixation consisted of one 1.8 mm tensioned transverse wire and two hydroxyapatite half pins secured to a full ring (Fig. 2).
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