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In all measurements, 1 mL of particle suspensions was employed and placed in a 10 mm × 10 mm quartz cuvette.
Spectrophotometric measurements of absorbance at 420, 520, and 620 nm were made using a 1 mm quartz cuvette.
The CD spectra of proteins were recorded with JASCO-J810 in a 1 mm quartz cuvette with a scan speed of 600 nm/min.
At the third stage, the LB concentration was calculated from UV absorption of this solution in 1 mm quartz cuvette at 343 nm using the previously prepared calibration curve.
Samples in a 1 mm quartz cuvette were maintained at 25°C with a circulating water bath.
RNA purity was quantified using a spectrophotometer using a 10 mm quartz cuvette.
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Fluorescence lifetimes: Recorded using time-correlated single-photon counting (TCSPC) in 10 mm quartz cuvettes.
The samples were placed in 1 mm quartz cuvettes and the kinetics of fibril formation recorded every 5 s at 218 nm for 60 min.
The turbidity spectra of the suspensions were recorded with a spectrophotometer Agilent 8453 in the range of 400 600 nm using 10 mm quartz cuvettes.
A photon-counting steady-state SLM 8100 with a double-grating excitation monochromator, a single-grating emission monochromator, and a 450 W xenon lamp was used for all experiments, as were 4 × 4 mm quartz cuvettes.
The absorption of the nanoparticles in the ultraviolet-visible-near infrared region (UV-Vis-NIR) was determined with a Beckman Coulter DU 800 spectrophotometer using a 0.1-mm quartz cuvette.
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