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Since the active chemical cue is not known (see supplementary material for more information regarding this subject), this step was achieved by placing one specimen of N. glauca into a 1 L glass jar containing 750 mL of SSS-medium, a 75 mm × 26 mm piece of fly screen made out of cotton (Tesa SE, Germany) for the notonectid to rest on, and five adult D. longicephala as food.

A 5 × 5 mm piece was used without any sample preparation.

A 150 × 150 × 525 mm notched beam for a three point bending test and a 150 × 300 mm piece for a Brazilian splitting cylinder test were defined as the standard cases (ACI and ASTM, Gerstle 2010).

Degradation of filter paper was measured by first cutting a 5 × 5 mm piece of filter paper #1 (Whatman, Kent, UK) with a pair of sharp scissors whereafter it was submerged in 80 μl of 1.25x McIlvaine Buffer (McIlvaine 1921) at the desired pH.

The explants (≈8 mm piece of rhizomatous stem from nodal portion containing axillary shoot bud) were cultured on MS (Murashige and Skoog 1962) basal medium containing 3.0% (w/v) sucrose and various concentration and combination of cytokinin [6-benzylaminopurine (BAP), Kinetin (KIN)] and Aloe vera leaf gel (AvG).

A 3 × 5 mm piece of sponge (Espongostan Film, Ferrosan A/S, Copenhagen, Denmark) soaked with MMC was placed under the conjunctival flap over the scleral bed in trabeculectomy and over the plate in sub-Tenon space in Ahmed valve for 2 min.

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Briefly, dissected brains were coronally cut into 2 mm pieces.

Remaining cornea stroma was cut into small 1 2 mm pieces and placed onto 35 mm Primaria Culture dishes (BD Biosciences, Franklin Lake, NJ).

Approximately 1.2 × 1.2 × 1.2 mm pieces from lungs frozen in OCT were thawed and washed 3 times in PBS and the lung piece was weighed.

Bone fragments were washed with phosphate buffered saline (PBS) and serum-free DMEM growth medium and cut into 2 3 mm pieces.

Cleaned roots and leaves were cut into 1 mm pieces and ball-milled for 8 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany).

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