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Clinically, the detection of the sentinel lymph node using ICG was restricted to 5 mm of tissue depth in oropharyngeal cancer patients [30].
Open image in new window Fig. 1 A 1.5 cm longitudinal incision was made with a number 21 scalpel, leaving 10 mm of tissue from the internal meniscal border.
Small pieces (5 × 2 × 1 mm) of tissue were obtained from the advancing margins of the diseased roots, surface-sterilized with 0.5% NaClO for 1 min, plated on potato dextrose agar (PDA) supplemented with 100 ppm streptomycin sulfate and 10 ppm benomyl for inhibition of growth of bacteria and other fungi, and incubated at room temperature (24 30 °C).
Total genomic DNA extracts were prepared from small pieces (≤1 mm) of tissue using the NucleoSpin® 96 Tissue kit (Macherey-Nagel Duren, Germany), following the manufacturer's protocols.
Their results showed a significant increase in the number of gap junctions per mm of tissue and an increase in the rate of rise of pressure in the estrogen treated rats.
Each lane was loaded with an equal tissue volume of 30 nL (5 mm of tissue).
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A similar procedure was performed at 1 and 7 days post-injury, and the tissue of the injury site plus 1-mm of tissue rostral and caudal to the injury site was collected and placed immediately into liquid nitrogen.
For example, a spectral data set collected from a 5 mm × 5 mm section of tissue, with 25 μm × 25 μm pixels, contains 40,000 individual infrared spectra, which may exceed 300 MB of memory.
These were microarrayed in a series of 24 blocks using 0.6 mm cylinders of tissue.
Briefly, a 2 mm strip of tissue was cut in the median of the P2 leaflet from the free edge to the annulus.
Using a steel Menghini needle, a representative 1.6 mm core of tissue was taken from each donor block and arrayed into the recipient wax block.
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