Reaction mixtures (200 μL total volume) of PbTx-6 (200 μM) were incubated with aliquots of 50 mM equivalents of individual nucleosides prepared in 100 mM phosphate buffer at pH 7.4.
Before the dip-coating process, the forewings (50 to 55 mm in length) of individual cicada were rinsed using ethyl alcohol and deionized water to remove contaminant and dried at room temperature.
Then, all images were normalized to the standard Montreal Neurological Institute (MNI) template (resampled into 3 × 3 × 3 mm) via parameters of individual structural image spatial normalization based on unified segmentation [ 29].
The 'apparent effects' of the observed genotypes MM, Mm and mm at marker i are the means of the observed phenotypes of individuals being MM, Mm and mm at marker i, respectively.
Multistudy, whole-brain, PPI-based contrast was performed on spatially smoothed (Gaussian filter: full-width at half-maximum=6 mm) volume time course files of individual subjects to identify the regions that showed the most significant beta values, indicating the strongest functional connectivity.
For visualization purposes only, the multistudy maps in Figs 2 and 3, and in Supplementary Figs 2 and 3 were created from spatially smoothed (Gaussian filter: full-width at half-maximum=9 mm) volume time course files of individual subjects from each experiment.
The most southern stand recorded the lowest sunshine hours (1,631 hours), mean annual temperature (10.5 C) and annual rainfall (770 mm) (a full summary of individual stands is contained in Additional file 1).
This observed pathway redundancy' underlines the necessity of comprehensive genetic approaches to make informed decisions on how to employ future targeted molecular therapies most effectively for the benefit of individual MM patients.
9 The PDF files of each report were presented to three scientists (GDC, ANN, MM), who were unaware of individual retraction status and asked to refrain from finding this out.
The diversity of mutations affecting different nodes of a particular signaling network appears to be an intrinsic feature of individual MM samples, and the elucidation of intra- as well as interindividual redundancy in mutations that affect survival pathways will help to better tailor targeted therapeutic strategies to the specific needs of the MM patient.
