Amperometric signals at 100 mM of glucose were 41.17 and 32.27 μA under aerobic and anaerobic conditions, respectively.
A linear relationship was observed between decrease in the photocurrent (the metabolism response) and the concentration to less than 60 mM of glucose (r=0.990).
Upon addition of glucose to the nanochannel enzyme system, the current response had a calibration range span from 0.005 to 2 mM of glucose concentration.
A linear range of 0 10 mM of glucose was demonstrated, which exhibits a high sensitivity as far as performance per immobilised GOx molecule is concerned.
Amperometric analysis showed that the sensitivity of CuNi-NGr based electrode was 7143 μA.mM−1.cm−2 at low (0.01 μM to 1 mM), and 1030 μA.mM−1.cm−2 at high concentrations (2 to 20 mM) of glucose.
It was found that the addition of 0.2 mM ascorbic acid, 0.1 mM acetaminophen, and 0.5 mM uric acid to 2 mM of glucose solution did not cause any impact on the response of the biosensor (Table 1).
Apparent K M and V max value of the purified GluDH-BA were determined using various substrate concentrations (10-250 mM of glucose) at a fixed concentration of the acceptor (0.5 mM of NAD+ or NADP+).
MM contained per 1 L: 200 mL 5 × M9 minimal salts (BD Difco) supplemented with 50 mM of glucose, 2 mM of MgSO4, 0.1 mM of CaCl2, 15 mg of thiamine, and 0.30 mg of selenite.
For the glucose assay using GOx/HRP format, we obtained the limit of detection (LOD ∼0.3 mM) and the limit of quantification (LOQ ∼0.9 mM) values in the dynamic detection range from 0.3 to 8.0 mM of glucose.
It has a detection limit about 100 μM (1.8 mg/dl), and a sensitivity about 58 MHz per 1 mM of glucose and exhibited a good linearity in human blood glucose level.
A loopful of this 48 h-culture was transferred to 25 mL of Yeast Nitrogen Medium (YNB; BioChemika Sigma Aldrich, Saint-Quentin Fallavier, France) supplemented with 30 mM of glucose (Sigma Aldrich) (YNB-Glc 30 mM) and incubated for 16 h at 37°C without shaking.
