The PCR mixture contained 0.2 mM of each dNTP, 1.5 mM MgCl2, 0.2 μM of each primer.
Each 25 μl PCR contained 5 μl of extracted DNA, 1 × buffer, 2.5 mM MgCl2, 1 mM of each primer, 1 mM mixed dNTPs and 0.2 μl AmpliTaq Gold enzyme (Applied Biosystems).
The fine earth fraction (< 2 mm) of each soil was characterized for a range of basic soil physical and chemical properties.
Evaluation was limited to the proximal 35 mm of each renal artery.
In 92% of scans, measurements were within 5 mm of each other.
The apical 3 mm of each tooth was sectioned and pulverized.
The reactions contained 100 ng of genomic DNA, 0.25 mM of each primer, 2 mM of each dNTP, 1.50 U Taq DNA polymerase (New England Biolabs, Cambridge, MA, USA), and 1× PCR buffer (New England Biolabs, Cambridge, MA, USA).
The PCR reaction mixture of 20 μl consisted of 10 ng of rice genomic DNA, 0.15 mM of each dNTP, 0.15 mM of each primer, 2 μl of 10 × PCR buffer and 1 unit of Taq polymerase.
Open image in new window Fig. 2 Dimensions (in mm) of each steel decking profile (t sd = 0.75 mm).
The apical 3 mm of each root were collected and placed in 50-ml test tubes containing liquid nitrogen (Praxair, Danbury, CT).
After being anesthetized the body weight (g) and total lengths (mm) of each fish were measured.
