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Stock solution, 20 mM of compounds were prepared in 100% DMSO and final concentration of DMSO at 30 μM is 0.15%.After 48 hrs, 200 μL MTT (0.5 mg/mL) was added to each well and incubated further for 4 hrs.
Concentrated stock solutions (1 mM) of compounds were prepared in water.
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In order to directly access the small intestine, 2.4 mM of compound 2A was perfused through the small intestine of an anesthetized rat for 12 minutes.
To obtain ligand complexes, AfNMT was incubated with 10 mM of compound.
The diluted protein solution (1 mg/ml) was incubated at room temperature for 2 h with 15 mM of compound 5 and then concentrated to 13.5 mg/ml.
4 mM solutions of compounds in DMSO were diluted 100 times with medium and added to cells to a final concentration of 20 μM (40 μL of cell culture plus 40 μL of compound solution, final concentration of DMSO - 0.5%).
Forty-eight 300 mm cubes of compound concrete and seventy-two 100 mm cubes of FC or demolished concrete alone were subjected to freeze-thaw cycling, and the compressive and splitting-loading failure strength of the frozen and thawed specimens was measured.
Volatile organic compounds with a volume ratio up to 1 10 0.1 mm of the compound to 1 m3 air; 0.1 parts-per-billion; 0.1 ppb) can be measured.
To test if a compound was a substrate for activated SAMHD1, 1 mM of the compound plus 200 μM GTP was included.
To determine whether a compound was both a substrate and an allosteric activator of SAMHD1, only 1 mM of the compound of interest was included in the reaction.
In assessing the effects of metal ions and chemical compounds on the enzyme, the enzyme in 50 mM Tris HCl buffer (pH 8.0) was treated with 1 mM of each compound (except EDTA; 10 mM) for 1 h at 30°C and residual activity was measured.
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CEO of Professional Science Editing for Scientists @ prosciediting.com