Results show that all extracts exhibited broad spectrum (6 29 mm) of antibacterial activity on most of the tested organisms.
The assay buffer consisted of 50 mM HEPES pH 7.6, 15 mM MgCl2, 1 mM EDTA, and the following substrate and effector concentrations were used: 1 mM CDP and 3 mM ATP (control for presence of activity), 1 mM CDP and 175 μM dATP (control for inactivation by dATP), 1 mM ADP and 120 μM dGTP, 1 mM GDP and 250 μM TTP, and 1 mM CDP/UDP and 1 μM dATP.
An inhibitory zone with a diameter less than 10 mm corresponds to lack of activity (10 mm is the diameter of the cup).
At higher concentrations of CaCl2 (5-25 mM), the activity of the enzyme was slightly inhibited.
The diameter of zone (mm) of L-asparaginase activity, as indicated by the formation of pink colored zone around the well against the yellow background, was measured.
At an ADP concentration of 1.2 mM, activity of 637 ± 69 nmol/(min mg prot) was reported (18), similar to our measurements (Fig. 3 D).
The authors observed that at high levels of copper sulphate (0.8 mM) the activity of crude Lcc was significantly inhibited possibly due to the weak fungal growth.
Spr Cel8A activity was enhanced by 108 and 81 % with the ion Co2+ at 1 and 5 mM, respectively; whereas in the presence of the ion Cu2+ (1 mM), the activity of the enzyme increased by 67%%.
At the high concentrations of Cu2+ and Fe3+ (10 mM), the activity of rPoMan5A was reduced to almost 50%.
To evaluate the fungicidal activity of green and red Brazilian propolis against C. albicans, Sporothrix schenckii and Paracoccicoides brasiliensis, an inhibition zone of less than 15 mm was considered lack of activity and all extracts presented similar activity [42].
