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The activities of the luciferase were measured in total protein extracts from triplicate samples (collected with a 5 mm leaf puncher with same number of leaf discs in each sample) using Berthold Centro LB960 luminometer with the luciferase assay system following the manufacturer's instruction (Promega).
In the subsequent year (rainfall 630 mm), leaf area index in the burned woodland recovered to be about 10% less than the unburned plot, remotely sensed ET rose above that in the unburned plot and recharge was lower in the burned than unburned plot.
While under NaCl treatment of 40 and 120 mM, leaf numbers were increased to 68.1 and 62.5, respectively compared with un-inoculated plants samples.
a meristematic cells (MCs) originated from the mesophyll cells of a leaf explant (Scale bar 0.1 mm); leaf vein (LV); b a somatic proembryo (SPE) and a somatic embryo developed the bipolar structure with putative shoot apical meristem (SAM) and putative root meristem (RM) (Scale bar 0.5 mm).
Twenty-four dafterftransplantingtingrowthwth status and tissue density were examined through the plant height (cm), stem diameter (mm), leaf length (cm), leaf width (cm), leaf number (ea/plant), leaf diameter (mm), chlorophyll concentration (mg/g FW), root length (cm), fresh weight (g/plant), and dried weight (g/plant).
Ten days after inoculation, 8 mm leaf punches were randomly taken from eight N. benthamiana plants.
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Inflorescence: panicle, 2--8-branched; nascent inflorescence pendent, axis 1--2.5 cm, > 1 mm wide; bracts 8--15 mm, leaf-like, lanceolate; pedicel 2--5 mm.
> 1 mm wide; bracts 5--10 mm, leaf-like, lance-linear, acuminate, glabrous; pedicel 5--8 mm, nonglandular-hairy or not.
Inflorescence: panicle, 4--6-branched; nascent inflorescence pendent, axis 1.5--2.5 1.5--2.5 1.5--2.5bracms 8--20 mm, leaf-like, lanceolate, acuminate, glandular-hairy; pedicel 8--15 mm, glandular-hairy.
Inflorescence: panicle, 3--5-branched; nascent inflorescence pendent, axis 0.5--1 cm, > 1 mm wide, long-glandular-hairy; bracts 5--10 mm, leaf-like, ovate, acute, glandular-hairy; pedicel 3--5 mm, glandular-hairy.
Inflorescence: panicle, 3--5-branched; nascent inflorescence axis pendent, 1--1.5 cm, > 1 mm wide, tomentose; bracts 5--15 mm, leaf-like, ovate to lance-ovate, canescent; pedicel 4--10 mm.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com