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The AC was entered at a plane of 90% scleral thickness using a 2.8 mm knife.
Limbal scleral-tunnel incision was made at 12 o'clock using 2.75 mm knife.
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Gaseous ammonia-pretreated switchgrass was prepared as for attritor milling (Fig. 6, 1 mm knife-milled, 150 160 °C, 1 h) and subjected to the above-mentioned milling methods.
These ammonia-treated samples were subsequently ball-milled with 1/4″ spherical steel beads from 0 to 67 h and compared to a similarly ball-milled but untreated switchgrass sample (91 % DM, initially 1 mm knife-milled).
> -wrap-foot> Particle size, surface area, crystallinity index (CrI) and coherent domain size of 1-mm knife-milled (KM) switchgrass subjected to further fine milling with and without 150 160 °C, 1 h, 10 wt% ammonia pretreatment.
To test if this was indeed so, we conducted a comparative study in which the moisture content of 1-mm knife-milled UT-5 switchgrass was adjusted to 8, 18 and 28%% prior to being placed in the 5-L Littleford Day horizontal pressure reactor for gaseous ammonia treatment.
To evaluate attritor milling as a means to further reduce the milling energy, we performed a series of milling experiments with an attritor mill using 1-mm knife-milled fall-harvested UT-4b switchgrass previously pretreated with 10%% gaseous ammonia at 150 160 °C for 1 h (98 % DM).
After embedding, sections of 60 nm each were cut using a Leica Ultracut E ultramicrotome equipped with a 2 mm diamond knife.
WS was coarsely cut to less than 2 mm by knife milling SM 100 (Retsch, Germany).
Semithin sections were cut using 6 mm glass knives on a Reichert-Jung Ultracut microtome.
Specimens were placed on a 10 mm diameter knife-edge circular support covered with thin rubber.
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