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Material and Methods: Two test conditions (bur immersed approximately 5 mm into a dye solution or completely immersed in dye solution) were conducted using potassium bichromate dye to simulate a fluid contaminant.
It consists in an embedding of the fragment in polyester resin polymerised by a peroxo organic hardener under low humidity conditions and sectioned with a diamond saw of thickness 0,1 mm into a 200 microns thick slice [13, 19].
These solutions were sprayed through a nozzle with a diameter of 0.4 mm into a high-pressure vessel filled with scCO2 to remove the organic solvents and precipitate the dried liposomes.
In contrast, for 150 μm tall channels, δ reached 3 mm into a 0.1 mm wide channel and 5.5 mm into a 1 mm wide channel.
The center plug protruded 0.5 mm into a milled depression in the bone cortex.
The model was constructed by implanting a short-stem prosthesis (Metha, modular stem, size 2, 135°, neutral) into a left medium-sized Sawbone femur (model 1121-69; Sawbones Pacific Research Laboratories, Vashon, WA), and an acetabular cup (Screwring SC, size 52 mm) into a left medium-sized hemipelvis (model 1305-9; Sawbones).
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The hairpin itself is a 1-mm thick copper strip, with a width of 5 mm, bent into a U shape of length 23 mm and plate separation of 5 mm.
A homemade PVC-coated concentric spinneret was prepared by inserting a metal concentric spinneret consisting of two stainless steel tubes (with inner diameters of 0.84 and 0.21 mm, respectively) into a PVC tube (inner diameter 1.0 mm, length 30 mm).
A typical titration consisted of injecting 5 10 μl aliquots of 1 5 mM peptide into a solution of 50 200 mM dSfmbt-4MBT protein at time intervals of 5 min to ensure that the titration peak returned to the baseline.
Using a bulb-headed cannula inserted 10 mm into the trachea, a suspension containing 5, 20, or 50 μg particles, respectively, in 50 μL pyrogene-free distilled water was instilled, followed by 100 μL air.
Eight hours after transfection, cells were transferred from a 60-mm dish into a 150-mm dish and selected for 10 days in the presence of 0.9 mg ml−1 of G418 (Life Technologies).
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